Université des Sciences de la Santé

  • Thematic field of study: Species diversity and insecticide Resistance in ANOPHELES in Libreville
  • Supervisor(s): Prof Marielle Bouyoy Akotet, Prof Patricia Mawili Mboumba, Dr Noe Patrick Mbondoukwe, Dr Ornella Mbang Nguema, Dr Jacques Mari Ndong Ngomo.
  • Contact details: dpmawili@gmail.com

 

Summary of Hosting Lab

The activities of the Joint Research Unit on Infectious Agents and Associated Pathologies focus on the genetic diversity of pathogens. Studies on factors influencing the expression of known or emerging parasites, bacteria and viruses as well as on vectors behavioural changes are conducted in urban areas. Vectors identification and distribution in these areas are performed.

Within JRAIP, a team is working on molecular characterization using conventional PCR, real-time PCR and RFLP-PCR.

Our unit is a research unit based at the University of Health Sciences in which students are introduced to basic, clinical and operational research; and carried out their research work.

Project Description

Currently, our team performs an inventory of potential vectors (Aedes and Anopheles) in urban areas, notably in the districts of Libreville. Therefore, the present project will be focused on the analysis of the species diversity of Anopheles. The objective is to identify using PCR
techniques Anopheles species and to detect the Kdr mutation. Collection of the larva will be performed in breeding sites close to households.

Publications

Ndong Ngomo JM et al. Drug Resistance Molecular Markers of Plasmodium falciparum and Severity of Malaria in Febrile Children in the Sentinel Site for Malaria Surveillance of Melen in Gabon: Additional Data from the Plasmodium Diversity Network African Network. Trop Med
Infect Dis. 2023 Mar 23;8(4):184.

Mbang Nguema O et al. Variations of Glossina sp. and trypanosome species frequency within different habitats in a sleeping sickness focus, Gabon. J Infect Dev Ctries. 2019 Jan 31;13(1):67-72. doi: 10.3855/jidc.9654.

Mbang Nguema OA et al. High Frequency of Trypanosoma congolense Savannah Type (Kinetoplastida: Trypanosomatidae) Among Tsetse Flies (Diptera: Glossinidae) in a Historic Trypanosoma Foci in North-Eastern Gabon: Preliminary Study [corrected]. J Med Entomol. 2016
Jul;53(4):945-948. doi: 10.1093/jme/tjw047.

Jean-Romain Mourou et al. Malaria transmission in Libreville: results of a one year survey. Malar J. 2012 Feb 9:11:40. doi: 10.1186/1475-2875-11-40.

Jean-Romain Mourou et al. Malaria transmission and insecticide resistance of Anopheles gambiae in Libreville and Port-Gentil, Gabon. Malar J. . 2010 Nov 11:9:321. doi: 10.1186/1475-2875-9-321.

 

Major Lab techniques

Larva sampling; Anopheles and Aedes adults microscopic identification, Nucleic acid extraction, qPCR, results analysis and interpretation (basic data analysis), scientific communication.

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